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Recombinant human PPCS protein

Recombinant human PPCS protein

Item no.	NKM-ATGP1064-20ug
Manufacturer	NKMAX
Amount	20ug
Category	Peptides & Proteins Recombinant Proteins SDS-PAGE
Type	Proteins Recombinant
Applications	SDS-PAGE
Specific against	other
Conjugate/Tag	His-Tag
Purity	> 95% by SDS-PAGE
NCBI	NP_078940
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Phosphopantothenate--cysteine ligase, COAB, PPC synthetase
Available
Antigen Species	Human
Bioassay Procedure	MGSSHHHHHH SSGLVPRGSH MAEMDPVAEF PQPPGAARWA EVMARFAARL GAQGRRVVLV TSGGTKVPLE ARPVRFLDNF SSGRRGATSA EAFLAAGYGV LFLYRARSAF PYAHRFPPQT WLSALRPSGP ALSGLLSLEA EENALPGFAE ALRSYQEAAA AGTFLAVEFT TLADYLHLLQ AAAQALNPLG PSAMFYLAAA VSDFYVPVSE MPEHKIQSSG GPLQITMKMV PKLLSPLVKD WAPKAFIISF KLETDPAIVI NRARKALEIY QHQVVVANIL ESRQSFVFIV TKDSETKLLL SEEEIEKGVE IEEKIVDNLQ SRHTAFIGDR N
Concentration	1mg/ml (determined by Bradford assay)
Description	PPCS, also known as phosphopantothenate--cysteine ligase, catalyzes the first step in the biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5), which is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine. Recombinant human PPCS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Domain	1-311aa
Expression System	E.coli
Formulation	Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Gene Category	Enzymes & Proteases
General References	Manoj N. et al. (2003) Structure. 11 : 927-936.; ; Christina Spry. et al. (2009) J Biol Chem. 284: 24904-24913.
Molecular Weight	36.1 kDa (331aa) confirmed by MALDI-TOF

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 20ug

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Delivery expected until 10/4/2024

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