Comparison

Recombinant human RHOA protein

Item no. NKM-ATGP0849-500ug
Manufacturer NKMAX
Amount 500ug
Category
Type Proteins Recombinant
Applications SDS-PAGE
Specific against other
Conjugate/Tag His-Tag
Purity > 90% by SDS-PAGE
NCBI NP_001655
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias RHOA,
Available
Antigen Species
Human
Concentration
1mg/ml (determined by Bradford assay)
Description
RhoA belongs to the small GTPase superfamily, Rho family, proteins involved in the regulation and timing of cell division. This protein is a small GTPase protein to regulate the actin cytoskeleton in the formation of stress fibers and acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila). Recombinant human RhoA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Expression System
E.coli
Formulation
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Gene Category
Cancer
General References
Madaule P. et al. (1985) Cell. 41:31-40; ; Garrett M.D. et al. (1989) J. Biol. Chem. 264:10-13
Molecular Weight
25.7 kDa (227aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 500ug
Available: In stock
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