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Recombinant human PHPT1 protein

Recombinant human PHPT1 protein

Item no.	NKM-ATGP0751-20ug
Manufacturer	NKMAX
Amount	20ug
Category	Peptides & Proteins Recombinant Proteins SDS-PAGE
Type	Proteins Recombinant
Applications	SDS-PAGE
Specific against	other
Conjugate/Tag	His-Tag
Purity	> 95% by SDS-PAGE
NCBI	NP_054891
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	14 kDa phosphohistidine phosphatase, bA216L13.10, CGI-202, DKFZp564M173, HSPC141, PHP14, RP11-216L13.10
Available
Antigen Species	Human
Bioassay Procedure	MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
Concentration	0.5mg/ml (determined by Bradford assay)
Description	PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Domain	1-125aa
Expression System	E.coli
Formulation	Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol
Gene Category	Enzymes & Proteases
General References	Srivastava S., et al. (2008) Proc Natl Acad Sci u S A. 105(38):14442-6.; ; van Vlierberghe P., et al. (2006) Leukemia. 20(7):1245-53.
Molecular Weight	15.9 kDa (145aa) confirmed by MALDI-TOF

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 20ug

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Delivery expected until 8/30/2024

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