Manufacturer |
NKMAX
|
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Applications |
SDS-PAGE |
Amount |
500ug |
Item no. |
NKM-ATGP0670-500ug |
Targets |
THRB |
Conjugate/Tag |
His-Tag |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Alias |
P4HB, Prolyl 4-hydroxylase subunit beta, P4Hbeta, PDI, PDIA1, PHD, PO4DB, PO4HB, ERBA2L, Prolyl 4-hydroxylase subunit beta |
Purity |
> 90% by SDS-PAGE |
Antigen Species |
Human |
Concentration |
1mg/ml (determined by Bradford assay) |
Description |
P4HB, prolyl 4 hydroxylase subunit beta, is a multifunctional and highly abundant enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This protein is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Recombinant human P4HB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. |
Expression System |
E.coli |
Formulation |
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol |
Gene Category |
Heat Shock Proteins |
General References |
Pfander D., et al. (2006) Am J Pathol. 169(2):491-502.; ; Kivirikko KI., (1991) J Hepatol. 13 Suppl 3:S2-7.; ; |
Molecular Weight |
57.5 kDa (512aa) confirmed by MALDI-TOF |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.