Item no. |
NKM-ATGP0602-500ug |
Manufacturer |
NKMAX
|
Amount |
500ug |
Category |
|
Type |
Proteins Recombinant |
Applications |
SDS-PAGE |
Specific against |
other |
Conjugate/Tag |
His-Tag |
Purity |
> 85% by SDS-PAGE |
NCBI |
NP_001001522 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Transgelin, TAGLN, Transgelin, SM22, SMCC, TAGLN1, WS3-10, Transgelin, SM 22, SM22 alpha, SMCC, Smooth muscle protein 22-alpha, WS3 10 |
Available |
|
Antigen Species |
Human |
Concentration |
1mg/ml (determined by Bradford assay) |
Description |
TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein that belongs to the calponin family. This protein is expressed abundantly in fibroblasts and smooth muscle. It is involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. During embryogenesis, TAGLN is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. It is downregulated in several transformed cell lines, indicating that a reduction of TAGLN expression may be an early indicator of the onset of transformation. Recombinant human TAGLN protein, fused to his-tag at N-terminus was expressed in E. coli and purified by using conventional chromatography techniques |
Expression System |
E.coli |
Formulation |
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 1mM DTT |
Gene Category |
Cytoskeleton |
General References |
Kobayashi R., et al. (1994) Biochem Biophys Res Commun. 198(3):1275-80. ; ; Thweatt R., et al. (1992) Biochem Biophys Res Commun. 187(1):1-7. ; ; |
Molecular Weight |
24.8 kDa (221aa) confirmed by MALDI-TOF |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.