Item no. |
LDR-R5255-100ug |
Manufacturer |
ACROBiosystems
|
Amount |
100 ug |
Category |
|
Type |
Proteins Recombinant |
Format |
Powder |
Specific against |
Rabbit |
Host |
HEK293 |
Conjugate/Tag |
Unconjugated, Fc |
Purity |
90% |
Citations |
(1) Yamamoto T, et al., 1984, Cell 39 (1): 27-38. (2) Nykjaer A, Willnow TE, 2002, Trends Cell Biol. 12 (6): 273-80. (3) Barrett P.H., Watts G.F. 2002, Atherosclerosis. Supplements. 2: 1-4. (4) Nomura S. et al., 2004, Gene. Therapy. 11: 1540-1548. |
ECLASS 5.1 |
34160400 |
ECLASS 6.1 |
34160400 |
ECLASS 8.0 |
42020190 |
ECLASS 9.0 |
42020190 |
ECLASS 10.0.1 |
32160409 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
LDLR,FH,FHC,LDLCQ2 |
Available |
|
Manufacturers Category |
Protein / Bio-Markers & CD Antigens |
Description |
Rabbit LDL R, Fc Tag (LDR-R5255) is expressed from human 293 cells (HEK293). It contains AA Ala 9 - Gly 765 (Accession # P20063-1). |
Molecule |
LDL R |
Exp Region |
Ala 9 - Gly 765 |
Storage |
-20℃ |
Shipping |
RT |
Stability |
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Molecular Weight |
109.2 kDa |
Characteristics |
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 109.2 kDa. The protein migrates as 120-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Endotoxin |
1.0 EU per μg |
Buffer |
Tris with Glycine, Arginine and NaCl, pH7.5 |
Background |
Low-Density Lipoprotein (LDL) Receptor is also known as LDLR, FH, FHC, LDLCQ2, and is a mosaic protein of ~840 amino acids (after removal of signal peptide) that mediates the endocytosis of cholesterol-rich LDL. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). It belongs to the Low density lipoprotein receptor gene family. LDL receptor complexes are present in clathrin-coated pits (or buds) on the cell surface, which when bound to LDL-cholesterol via adaptin, are pinched off to form clathrin-coated vesicles inside the cell. This allows LDL-cholesterol to be bound and internalized in a process known as endocytosis and prevents the LDL just diffusing around the membrane surface. This occurs in all nucleated cells (not erythrocytes), but mainly in the liver which removes ~70% of LDL from the circulation. Synthesis of receptors in the cell is regulated by the level of free intracellular cholesterol; if it is in excess for the needs of the cell then the transcription of the receptor gene will be inhibited. LDL receptors are translated by ribosomes on the endoplasmic reticulum and are modified by the Golgi apparatus before travelling in vesicles to the cell surface. LDL is directly involved in the development of atherosclerosis, due to accumulation of LDL-cholesterol in the blood. Atherosclerosis is the process responsible for the majority of cardiovascular diseases. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.