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Biotinylated Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein, His,Avitag™&Tag Free

Biotinylated Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein, His,Avitag™&Tag Free

Item no.	IT1-H82W1-200ug
Manufacturer	ACROBiosystems
Amount	100 ug x 2
Quantity options	100 ug x 2 25 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Powder
Specific against	Human (Homo sapiens)
Host	HEK293
Conjugate/Tag	Biotin, HIS, Avi
Purity	95%
Citations	(1) May AE., et al.2000, Blood., 96(2), 506-13. (2) Luo BH., et al.2007, Annu Rev Immunol., 25, 619-47. (3) akada, Y., et al. 2007 Genome Biol., 8(5), 215.
NCBI	NP 000876.3 (ITGA4) , NP_002202.2 (ITGB1)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Integrin alpha 4 beta 1,ITGA4&ITGB1
Shipping condition	Room temperature
Available
Manufacturer - Category	Protein / MABSol® Biotin Labeled Proteins
Manufacturer - Conjugate / Tag	Biotin-labeled, C-10xHis & C-Avi \| Native
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	113.2 kDa (ITGA4) & 83.7 kDa (ITGB1)
Description	Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His, Avitag&Tag Free (IT1-H82W1) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1) (Accession # P13612-1 (ITGA4) & P05556-1 (ITGB1)).
Background	Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. Integrin alpha 4 beta 1(Alpha-4/beta-1) is receptors for fibronectin. Integrin alpha-4/beta-1 is a receptor for VCAM1 and recognizes the sequence Q-I-D-S in VCAM1.
Molecule	Integrin alpha 4 beta 1
Exp Region	Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1)
Characteristics	Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His, Avitag&Tag Free, produced by co-expression of ITGA4 and ITGB1, has a calculated MW of 113.2 kDa (ITGA4) and 83.7 kDa (ITGB1). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The ITGA4 subunit is composed of a heavy chain (Tyr 34 - Arg 591, calculated MW 61.1 kDa) and a light chain (Ser 592 - Thr 977, calculated MW 52.1 kDa). Consequently ITGA4 migrates as 65-80 kDa, and ITGB1 as 90-150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin	1.0 EU per μg
Buffer	50 mM Tris, 150 mM NaCl, pH7.5
Stability	● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution.For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

IT1-H82W1-200ug-datasheet.pdf

IT1-H82W1-200ug-safety-datasheet.pdf