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Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein, His Tag&Tag Free

Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein, His Tag&Tag Free

Item no.	IT1-H52W5-1mg
Manufacturer	ACROBiosystems
Amount	200 ug x 5
Quantity options	100 ug 200 ug x 5
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Powder
Specific against	Human (Homo sapiens)
Host	HEK293
Conjugate/Tag	Unconjugated, HIS
Purity	95%
Citations	(1) Wei Xia., et al., 2014, Proc Natl Acad Sci U S A. 111(50): 17863-17868. (2) Masamichi N., et al., 2012, J Cell Biol. 197(1): 131-140. (3) Takagi J., et al., 2003, EMBO J. 22(18):4607-15.
NCBI	NP_002196.4 (ITGA5) , NP_002202.2 (ITGB1)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Integrin alpha 5 beta 1,ITGA5&ITGB1
Shipping condition	Room temperature
Available
Manufacturer - Category	Protein / Bio-Markers & CD Antigens
Manufacturer - Conjugate / Tag	Unconjugated, C-10xHis \| Native
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	110.7 kDa (ITGA5) & 83.7 kDa (ITGB1)
Description	Human ITGA5&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52W5) is expressed from human 293 cells (HEK293). It contains AA Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1) (Accession # P08648-1 (ITGA5) & P05556-1 (ITGB1)).
Background	Integrin alpha 5 beta 1 / Integrin α5β1, which is a major cellular receptor for the extracellular matrix protein fibronectin and plays a fundamental role during mammalian development., is composed of α5 (ITGA5/CD49e) and β1 (ITGB1/CD29) subunits. Integrins are adhesion receptors that transmit bidirectional signals across the plasma membrane. The Integrin alpha 5 beta 1 and its primary extracellular matrix ligand fibronectin (Fn) are of great biological importance.
Molecule	Integrin alpha 5 beta 1
Exp Region	Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1)
Characteristics	Human ITGA5&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA5 and ITGB1, has a calculated MW of 110.7 kDa (ITGA5) and 83.7 kDa (ITGB1). Subunit ITGA5 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-150 kDa (ITGA5) and 100-120 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker respectively due to glycosylation.
Endotoxin	1.0 EU per μg
Buffer	50 mM Tris, 150 mM NaCl, pH7.5
Stability	● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution.For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

IT1-H52W5-1mg-datasheet.pdf

IT1-H52W5-1mg-safety-datasheet.pdf