Comparison

Recombinant Mycobacterium tuberculosis Steroid C26-monooxygenase(cyp125)

Item no. CSB-YP351308MVZ-20
Manufacturer Cusabio
Amount 20ug
Quantity options 1mg 10ug 100ug 20ug 200ug 50ug 500ug
Category
Type Proteins Recombinant
Format Liquid or Lyophilized powder
Specific against other
Host Yeast
Purity Greater than 90% as determined by SDS-PAGE.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Available
Research Areas
Others
Uniprot ID
P9WPP1
Gene Names
cyp125
Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
AA Sequence
MSWNHQSVEIAVRRTTVPSPNLPPGFDFTDPAIYA ERLPVAEFAELRSAAPIWWNGQDPGKGGGFHDGGF WAITKLNDVKEISRHSDVFSSYENGVIPRFKNDIA REDIEVQRFVMLNMDAPHHTRLRKIISRGFTPRAV GRLHDELQERAQKIAAEAAAAGSGDFVEQVSCELP LQAIAGLLGVPQEDRGKLFHWSNEMTGNEDPEYAH IDPKASSAELIGYAMKMAEEKAKNPADDIVTQLIQ ADIDGEKLSDDEFGFFVVMLAVAGNETTRNSITQG MMAFAEHPDQWELYKKVRPETAADEIVRWATPVTA FQRTALRDYELSGVQIKKGQRVVMFYRSANFDEEV FQDPFTFNILRNPNPHVGFGGTGAHYCIGANLARM TINLIFNAVADHMPDLKPISAPERLRSGWLNGIKH WQVDYTGRCPVAH
Expression Region
1-433aa
Sequence Info
Full Length
Source
Yeast
Tag Info
N-terminal 6xHis-tagged
MW
50.4 kDa
Alternative Name(s)
Cholest-4-en-3-one 26-monooxygenaseCytochrome P450 125Steroid C27-monooxygenase
Relevance
Catalyzes the C-27 hydroxylation of cholest-4-en-3-one and cholesterol and subsequently oxidizes the alcohol of the former to the cholest-4-en-3-one-27-oic acid via the aldehyde intermediate. Not required to incorporate the cholesterol side-chain carbon atoms into cellular lipids.
Reference
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.Nature 393:537-544(1998)
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection
Protein Families
Cytochrome P450 family
Tag Information
N-terminal 6xHis-tagged

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 20ug
Available: In stock
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