Item no. |
CSB-YP025857HU-20 |
Manufacturer |
Cusabio
|
Amount |
20ug |
Category |
|
Type |
Proteins Recombinant |
Format |
Liquid or Lyophilized powder |
Specific against |
other |
Purity |
Greater than 90% as determined by SDS-PAGE. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Available |
|
Research Areas |
Cancer |
Uniprot ID |
P08670 |
Gene Names |
VIM |
Organism |
Homo sapiens (Human) |
AA Sequence |
STRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTR TYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLR SSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVE LQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQ GKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERD NLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVD NASLARLDLERKVESLQEEIAFLKKLHEEEIQELQ AQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAA KNLQEAEEWYKSKFADLSEAANRNNDALRQAKQES TEYRRQVQSLTCEVDALKGTNESLERQMREMEENF AVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDL LNVKMALDIEIATYRKLLEGEESRISLPLPNFSSL NLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVIN ETSQHHDDLE |
Expression Region |
2-466aa |
Sequence Info |
Full Length of Mature Protein |
Source |
Yeast |
Tag Info |
N-terminal 6xHis-tagged |
MW |
55.5 kDa |
Relevance |
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. |
Reference |
Coding sequence and growth regulation of the human vimentin gene.Ferrari S., Battini R., Kaczmarek L., Rittling S., Calabretta B., de Riel J.K., Philiponis V., Wei J.-F., Baserga R.Mol. Cell. Biol. 6:3614-3620(1986) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. |
Involvement in disease |
Cataract 30, multiple types (CTRCT30) |
Subcellular Location |
Cytoplasm |
Protein Families |
Intermediate filament family |
Tissue Specificity |
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
Tag Information |
N-terminal 6xHis-tagged |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.