Item no. |
CSB-EP619866HU-20 |
Manufacturer |
Cusabio
|
Amount |
20ug |
Category |
|
Type |
Proteins Recombinant |
Format |
Liquid or Lyophilized powder |
Specific against |
Human |
Host |
E.coli |
Purity |
Greater than 90% as determined by SDS-PAGE. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Available |
|
Research Areas |
Cell Biology |
Target / Protein |
DCTN1 |
Biologically Active |
Not Test |
Expression System |
E.coli |
Species of origin |
Homo sapiens (Human) |
Uniprot ID |
Q14203 |
AA Sequence |
PSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKE LEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKE AKEALEAKERYMEEMADTADAIEMATLDKEMAEER AESLQQEVEALKERVDELTTDLEILKAEIEEKGSD GAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQE HVKLQKLMEKKNQELEVVRQQRERLQEELSQAEST IDELKEQVDAALGAEEMVEMLTDRNLNLEEKVREL RETVGDLEAMNEMNDELQENARETELELREQLDMA GARVREAQKRVEAAQETVADYQQTIKKYRQLTAHL QDVNRELTNQQEASVERQQQ |
Tag Info |
N-terminal 6xHis-SUMO-tagged |
Expression Region |
213-547 |
Protein Length |
Partial |
MW |
55.2 kDa |
Alternative Name(s) |
150 kDa dynein-associated polypeptide DAP-150 |
Relevance |
Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. |
Reference |
Human DCTN1: genomic structure and evaluation as a candidate for Alstrom syndrome.Collin G.B., Nishina P.M., Marshall J.D., Naggert J.K. Genomics 53:359-364(1998) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule |
Involvement in disease |
Neuronopathy, distal hereditary motor, 7B (HMN7B); Amyotrophic lateral sclerosis (ALS); Perry syndrome (PERRYS) |
Subcellular Location |
Cytoplasm, Cytoplasm, cytoskeleton, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole, Cytoplasm, cytoskeleton, spindle, Nucleus envelope, Cytoplasm, cell cortex |
Protein Families |
Dynactin 150 kDa subunit family |
Tissue Specificity |
Brain. |
Paythway |
SecretedExtracellularVesicles |
Tag Information |
N-terminal 6xHis-SUMO-tagged |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.