Recombinant Escherichia coli Polyphosphate kinase(ppk)

Cell Biology

Not Test

Escherichia coli (strain K12)

GQEKLYIEKELSWLSFNERVLQEAADKSNPLIERM RFLGIYSNNLDEFYKVRFAELKRRIIISEEQGSNS HSRHLLGKIQSRVLKADQEFDGLYNELLLEMARNQ IFLINERQLSVNQQNWLRHYFKQYLRQHITPILIN PDTDLVQFLKDDYTYLAVEIIRGDTIRYALLEIPS DKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIF KGFFDYDALNAYSMKMTRDAEYDLVHEMEASLMEL MSSSLKQRLTAEPVRFVYQRDMPNALVEVLREKLT ISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPL PRLRHIWFDKAQFRNGFDAIRERDVLLYYPYHTFE HVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSM IHAAHNGKKVTVVVELQARFDEEANIHWAKRLTEA GVHVIFSAPGLKIHAKLFLISRKENGEVVRYAHIG TGNFNEKTARLYTDYSLLTADARITNEVRRVFNFI ENPYRPVTFDYLMVSPQNSRRLLYEMVDREIANAQ QGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNL LVRGMCSLIPNLEGISDNIRAISIVDRYLEHDRVY IFENGGDKKVYLSSADWMTRNIDYRIEVATPLLDP RLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRG NRRKVRAQLAIYDYIKSLEQPE

2-688aa

87.7 kDa

Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate. Can form linear polymers of orthophosphate with chain lengths up to 1000 or more. Can use GTP instead of ATP, but the efficiency of GTP is 5% that of ATP. Also exhibits several other enzymatic activities, which include: ATP synthesis from polyP in the presence of excess ADP, general nucleoside-diphosphate kinase activity, linear guanosine 5'-tetraphosphate synthesis and autophosphorylation.

Greater than 85% as determined by SDS-PAGE.

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.

N-terminal 10xHis-tagged and C-terminal Myc-tagged