Manufacturer |
Cusabio
|
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Format |
Liquid or Lyophilized powder |
Amount |
20ug |
Host |
E.coli |
Item no. |
CSB-EP004561HU-20 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Purity |
Greater than 90% as determined by SDS-PAGE. |
Research Areas |
Neuroscience |
Uniprot ID |
P20810 |
Gene Names |
CAST |
Organism |
Homo sapiens (Human) |
AA Sequence |
MNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSN DAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGG ESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDK PSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPE VSDPMSSTYIEELGKREVTIPPKYRELLAKKEGIT GPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKT EKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTM SDQALEALSASLGTRQAEPELDLRSIKEVDEAKAK EEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEP EEKPKPRSESELIDELSEDFDRSECKEKPSKPTEK TEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLK GTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEK AKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLP KESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFE DAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKD LDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKA EHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTK KSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNT AKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPK DD |
Expression Region |
1-667aa |
Sequence Info |
Full Length of isoform 4 |
Source |
E.coli |
Tag Info |
N-terminal 6xHis-tagged |
MW |
75.9 kDa |
Alternative Name(s) |
Calpain inhibitor; Sperm BS-17 component |
Relevance |
Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmort tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. |
Reference |
cDNA cloning of human calpastatin sequence homology among human, pig, and rabbit calpastatins.Asada K., Ishino Y., Shimada M., Shimojo T., Endo M., Kimizuka F., Kato I., Maki M., Hatanaka M., Murachi T.J. Enzym. Inhib. 3:49-56(1989) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. |
Involvement in disease |
Peeling skin with leukonychia, acral punctate keratoses, cheilitis, and knuckle pads (PLACK) |
Protein Families |
Protease inhibitor I27 (calpastatin) family |
Tag Information |
N-terminal 6xHis-tagged |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.