Item no. |
BOS-PROTP61218 |
Manufacturer |
Boster
|
Amount |
20ug |
Category |
|
Type |
Proteins Recombinant |
Format |
Solution |
Specific against |
other |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
DNA-directed RNA polymerases I, II, and III subunit RPABC2;RNA polymerases I, II, and III subunit ABC2;DNA-directed RNA polymerase II subunit F;DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide;RPABC14.4;RPB14.4;RPB6 homolog;RPC15;POLR2F;POLRF; |
Available |
|
Contents |
The POLR2F solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT. |
Description |
POLR2F Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 150 amino acids (1-127) and having a molecular mass of 16.9kDa (Molecular size on SDS-PAGE will appear higher). POLR2F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
Gene name |
POLR2F |
Protein function |
DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds (By similarity). |
Purification |
Greater than 95% as determined by SDS-PAGE. |
Shipping |
Shipped with wet ice |
Short description |
POLR2F Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 150 amino acids (1-127) and having a molecular mass of 16.9kDa (Molecular size on SDS-PAGE will appear higher). POLR2F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
Storage |
Store in -20° C for long term storage. After reconstitution, store in 4° C for short term usage within a few days. Avoid freeze-thaw cycles. |
Subcellular localization |
Nucleus |
Format Information |
Sterile Filtered colorless solution. |
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