Item no. |
BA2-HM138-500UG |
Manufacturer |
KactusBio
|
Amount |
500 ug |
Category |
|
Type |
Proteins Recombinant |
Format |
Lyophilized |
Specific against |
SARS-CoV-2 |
Host |
HEK293 |
Conjugate/Tag |
HIS |
Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Formula |
Supplied as 0.22μm filtered solution in PBS (pH 7.4). |
Sequence |
Arg319-Phe541(G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417T, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H) |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Available |
|
Description |
Recombinant SARS-CoV-2 Spike RBD (Omicron BA.2.38) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417T, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H). |
Background |
The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein. |
Manufacturers Product Type |
Recombinant Protein |
Endotoxin |
Less than 1EU per μg by the LAL method. |
Molecular Weight |
The protein has a predicted MW of 26.07 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result. |
Stability and Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3-6 months after reconstitution.;2-8°C for 2-7 days after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Tag Information |
C-His |
Formulation |
Supplied as 0.22μm filtered solution in PBS (pH 7.4). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.