Comparison

Pfu-DNA Polymerase Recombinant

Item no. ANG-rAP-1061-100U
Manufacturer Angio-Proteomie
Amount 100 U
Quantity options 100 U 1 ea 2500 U 500 U
Category
Type Proteins Recombinant
Specific against other
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Available
Description
Product Name: Pfu-DNA Polymerase Recombinant
Synonyms: DNA polymerase, EC 2.7.7.7, Pfu polymerase, Pfu-DNA Polymerase.
Description: Pfu DNA Polymerase is a thermo-stable enzyme having a Mw of about 90kDa.
Pfu DNA Polymerase is derived from E. coli that and cloned from Pyrococcus furiosus strain Vc1 DSM3638. Pfu DNA Polymerase replicates DNA at 75C, catalyzing the polymerization of nucleotides into duplex DNA in the 5´ to 3´ direction in the existence of magnesium. Pfu DNA Polymerase possesses 3´ to 5´ exonuclease (proofreading) activity. Base misinsertions that take place during polymerization are swiftly removed by the proofreading activity of the polymerase. Therefore, Pfu DNA Polymerase is suggested for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended.
Uniprot Accesion Number: P61875
Amino Acid Sequence:
Source: Escherichia Coli.
Physical Appearance and Stability: Pfu DNA Polymerase although stable at 10C for 5 days, should be stored below -18C.Please prevent freeze-thaw cycles.
Formulation and Purity: 50mM Tris-HCl, pH 8.2, 1mM DTT, 0.1mM EDTA, 0.05% CHAPS and 50% glycerol.
Application: 1. Ideal for high-fidelity amplification. 2. 3'-5' exonuclease activity provides a low error rate. 3. One of the most thermostable DNA polymerases known. 4. Lack of extendase activity means no unwanted 3¿ overhangs. 5. Optimal for blunt-end PCR cloning. 6. Optimum temperature near 75C. 7. 95% active after 1-hour incubation at 98C.
Solubility:
Biological Activity:
Shipping Format and Condition: Lyophilized powder at room temperature.
Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 U
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close