Mouse Cluster Of Differentiation 320 (CD320) Protein

Cluster Of Differentiation 320 Protein is a recombinant Mouse protein expressed in E. coli.

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.

Calculated MW: 23.2 kDa
Observed MW: 33 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.

Mouse

Cluster Of Differentiation 320 (CD320)