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Recombinant Rat 5'-nucleotidase/CD73/NT5E Protein (His Tag)

Recombinant Rat 5'-nucleotidase/CD73/NT5E Protein (His Tag)

Item no.	80335-R08H-2
Manufacturer	Sino Biological
Amount	2 mg
Quantity options	1 mg 2 mg 4 mg 50 ug
Category	Oncology Food Safety Fungicides Neuroscience Molecular Targets for Neuroscience By Cell Type Carcinoma Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Rat
Host	Human
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	80335-R08H
Similar products	NT5E
Available
Manufacturer - Category	Protein
Molecule	NT5E
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the rat NT5E(Q66HL0) (Met1-Lys549) was expressed with a polyhistidine tag at the C-terminus.
Expressed Host	Human Cells
Purity	> 90 % as determined by SDS-PAGE
Bio-Activity	Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996). The specific activity is >15, 000 pmol/min/ug.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Trp 29
Molecule Mass	The recombinant rat NT5E comprises 532 amino acids and predicts a molecular mass of 59.4 kDa. The apparent molecular mass of the recombinant protein is approximately 59 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Accession	Q66HL0
Amino acid sequence	Met1-Lys549
TAG	C-His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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80335-R08H-2-datasheet.pdf

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