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Recombinant Human hCD83, Biotinylated

Recombinant Human hCD83, Biotinylated

Item no.	230-30074B-200
Manufacturer	Raybiotech
Amount	200 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Lyophilized powder
Applications	WB, IP, ELISA, Dot
Specific against	Human
Purity	>95% - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Available
Gene Symbols	CD83
Application Information	Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Storage/Stability	Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
Formulation	Lyophilized from a 0.2 ?m filtered solution in PBS
Expressed Region	Thr20-Glu144
Protein Name & Synonyms	hCD83
Expression System	Human Embryonic Kidney 293 Cells
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration (lot specific)	Determined by Pierce BCA protein assay kit
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Preservative	None
Conjugation/Tag Notes	N-terminal his-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method.
Molecular Weight (kDa)	Recombinant Human hCD83 has a calculated molecular mass of 15 kDa. Beside one minor 15-kDa protein band, one major enlarged protein band (~18 kDa) also presented in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Shipping	Ice packs
Conjugation	Biotin

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 200 ug

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Delivery expected until 9/20/2024

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