Item no. |
230-30074B-200 |
Manufacturer |
Raybiotech
|
Amount |
200 ug |
Category |
|
Type |
Proteins Recombinant |
Format |
Lyophilized powder |
Applications |
WB, IP, ELISA, Dot |
Specific against |
Human |
Purity |
>95% - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Available |
|
Gene Symbols |
CD83 |
Application Information |
Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc. |
Storage/Stability |
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles. |
Formulation |
Lyophilized from a 0.2 ?m filtered solution in PBS |
Expressed Region |
Thr20-Glu144 |
Protein Name & Synonyms |
hCD83 |
Expression System |
Human Embryonic Kidney 293 Cells |
Purification |
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
Concentration (lot specific) |
Determined by Pierce BCA protein assay kit |
Reconstitution |
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
Preservative |
None |
Conjugation/Tag Notes |
N-terminal his-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
Molecular Weight (kDa) |
Recombinant Human hCD83 has a calculated molecular mass of 15 kDa. Beside one minor 15-kDa protein band, one major enlarged protein band (~18 kDa) also presented in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
Shipping |
Ice packs |
Conjugation |
Biotin |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.