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Recombinant Human NT-proBNP, Biotinylated

Recombinant Human NT-proBNP, Biotinylated

Item no.	230-30019B-50
Manufacturer	Raybiotech
Amount	50 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Lyophilized powder
Applications	WB, IP, ELISA, Dot
Specific against	Human
Conjugate/Tag	His
Purity	>90% - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Available
Gene Symbols	NPPB
Application Information	ELISA, EIA, protein-protein interaction studies, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array
Storage/Stability	Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Formulation	Lyophilized from a 0.2 um filtered solution in PBS
Expressed Region	His27-Arg102
Protein Name & Synonyms	NT-proBNP, Natriuretic peptides B, Natriuretic Peptide B, Brain Type Natriuretic Peptide, Natriuretic Peptide Precursor B, Gamma-Brain Natriuretic Peptide, Natriuretic Protein, BNP
Expression System	Human Embryonic Kidney 293 Cells
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration (lot specific)	Determined by BCA protein assay kit (Thermo Scientific)
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level	Not determined
Preservative	None
Conjugation/Tag Notes	N-terminal 6x histidine tag and C-terminal mouse IgG Fc tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method.
Molecular Weight (kDa)	Recombinant human NT-proBNP has a calculated molecular mass of 35 kDa (including a C-terminal mouse IgG Fc tag, 27 kDa). Due to the abundant glycosylation, it migrates as approximately 40-50 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Shipping	Ice packs
Protein Type	Recombinant Protein
Conjugation	Biotin

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 ug

available

Delivery expected until 9/13/2024