Item no. |
230-30019B-50 |
Manufacturer |
Raybiotech
|
Amount |
50 ug |
Category |
|
Type |
Proteins Recombinant |
Format |
Lyophilized powder |
Applications |
WB, IP, ELISA, Dot |
Specific against |
Human |
Conjugate/Tag |
His |
Purity |
>90% - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Available |
|
Gene Symbols |
NPPB |
Application Information |
ELISA, EIA, protein-protein interaction studies, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array |
Storage/Stability |
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles. |
Formulation |
Lyophilized from a 0.2 um filtered solution in PBS |
Expressed Region |
His27-Arg102 |
Protein Name & Synonyms |
NT-proBNP, Natriuretic peptides B, Natriuretic Peptide B, Brain Type Natriuretic Peptide, Natriuretic Peptide Precursor B, Gamma-Brain Natriuretic Peptide, Natriuretic Protein, BNP |
Expression System |
Human Embryonic Kidney 293 Cells |
Purification |
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
Concentration (lot specific) |
Determined by BCA protein assay kit (Thermo Scientific) |
Reconstitution |
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
Endotoxin Level |
Not determined |
Preservative |
None |
Conjugation/Tag Notes |
N-terminal 6x histidine tag and C-terminal mouse IgG Fc tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
Molecular Weight (kDa) |
Recombinant human NT-proBNP has a calculated molecular mass of 35 kDa (including a C-terminal mouse IgG Fc tag, 27 kDa). Due to the abundant glycosylation, it migrates as approximately 40-50 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
Shipping |
Ice packs |
Protein Type |
Recombinant Protein |
Conjugation |
Biotin |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.