Manufacturer |
Sino Biological
|
Category |
|
Type |
Proteins |
Specific against |
Human |
Amount |
1mg |
Host |
Baculovirus-Infected Insect Cells |
Item no. |
10755-H20B-1 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Alias |
ASV, c-SRC, p60-Src, SRC1 |
Molecule |
SRC |
Synonym |
ASV, c-SRC, p60-Src, SRC1 |
Storage |
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
Protein Construction |
A DNA sequence encoding the human SRC (P12931-1) (Met 1-Leu 536) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
Expressed Host |
Baculovirus-Insect Cells |
Purity |
> 90 % as determined by SDS-PAGE |
Bio-Activity |
The specific activity was determined to be 27 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. |
Endotoxin |
< 1.0 EU per ug of the protein as determined by the LAL method |
Predicted N Terminal |
Met |
Molecule Mass |
The recombinant human SRC/GST chimera consists of 773 amino acids and has a calculated molecular mass of 87.7 KDa. It migrates as an approximately 81 KDa band in SDS-PAGE under non-reduced conditions. |
Formulation |
Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% gly, 0.3mM DTT |
Accession |
P12931-1 |
Amino acid sequence |
Met1-Leu536 |
TAG |
N-GST & His |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.