Manufacturer |
ProSpec
|
Category |
|
Type |
Cytokines and Growth Factors |
Specific against |
Human |
Amount |
5ug |
Item no. |
cyt-575-5ug |
eClass 6.1 |
32161000 |
eClass 9.0 |
42030690 |
Available |
|
Synonyms |
Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, MMIF, MIF |
Introduction |
The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. |
Description |
MIF human Recombinant was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques. Macrophage Inducing Factor Human Recombinant is a single, non-glycosylated, polypeptide chain containing 115 amino acids and having a molecular mass of 12 kDa. |
Source |
Escherichia Coli. |
Physical Appearance |
Sterile Filtered clear solution. |
Formulation |
1mg/ml solution containing 50mM Tris-HCl pH-8, 0.5mM DTT 10% glycerol. |
Purity |
Greater than 98.0% as determined by SDS-PAGE. |
Usage |
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
Amino acid sequence |
MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA. |
Storage |
Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.