Item no. |
cyt-211-5ug |
Manufacturer |
ProSpec
|
Amount |
5ug |
Category |
|
Type |
Cytokines and Growth Factors |
Specific against |
Human |
ECLASS 10.1 |
42030690 |
ECLASS 11.0 |
42030690 |
UNSPSC |
12352202 |
Similar products |
IL 4 |
Available |
|
Synonyms |
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra |
Introduction |
IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
Description |
Interleukin-4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The IL-4 is purified by proprietary chromatographic techniques. |
Source |
Escherichia Coli. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a0.2um filtered concentrated (1mg/ml) solution in PBS, pH 7.4. |
Purity |
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Usage |
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
Amino acid sequence |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Lys-Cys-Asp. |
Biological Activity |
The ED50 as determined by the dose-dependant stimulation of TF-1 cells is 0.2 ng/ml, corresponding to a Specific Activity of 5, 000, 000 IU/mg. |
Storage |
Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL4 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.