Item no. |
NKM-ATGP4125-50ug |
Manufacturer |
NKMAX
|
Amount |
50ug |
Applications |
SDS-PAGE |
Specific against |
Human |
Conjugate/Tag |
His-Tag |
Purity |
> 95% by SDS-PAGE |
Citations |
Millan, J.L. and W.H. Fishman (1995) Crit. Rev. Clin. Lab. Sci. 32:1-39.<br> Sara Sultana., et al, (2013) Mol Genet Metab. 109:282-288.<br> Sonia Di Mauro., et al, (2002) J Bone Miner Res. 17:1383-1391. |
NCBI |
NP_000469.3 |
Alias |
Aalkaline phosphatase, tissue-nonspecific isozyme isoform 1, Alkaline phosphatase liver/bone/kidney isozyme, Phosphoamidase, Phosphocreatine phosphatase, ALPL, AP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP |
Similar products |
ATGP4125-10ug, ATGP4125-20ug, ATGP4125-50ug, ATGP4125-100ug, ATGP4125-250ug, ATGP4125-500ug, ATGP4125-1mg, ATGP4125-010, ATGP4125-020, ATGP4125-050, ATGP4125-100, ATGP4125-250, ATGP4125-500, ATGP4125-01M |
Available |
|
Domain |
18-501aa |
Description |
ALPL, also known as alkaline phosphatase, tissue-nonspecific isozyme, is one of alkaline phosphatases. Alkaline phosphatase is metabolizes various phosphate compounds and plays a key role in skeletal mineralization and adaptive thermogenesis. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue-nonspecific). Expression of this protein is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. It plays an essential role in skeletal and dental mineralization via its ability to hydrolyze extracellular diphosphate, a potent mineralization inhibitor, to phosphate. It thereby promotes hydroxyapatite crystal formation and increases inorganic phosphate concentration. Recombinant human Alkaline Phosphatase/ALPL, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques. |
Concentration |
1mg/ml (determined by Absorbance at 280nm) |
Formulation |
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Bioactivity Comment Only |
Specific activity is > 40, 000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 37C. |
Manufacturers Gene Category |
Enzymes & Proteases, Cell Cycle |
Molecular Weight |
54.3kDa (493aa) |
Endotoxin |
< 1 EU per 1ug of protein (determined by LAL method) |
Bioactivity Comment and Pics |
Specific activity is > 40, 000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 37C. |
Bioassay Procedure |
LVPEKEK DPKYWRDQAQ ETLKYALELQ KLNTNVAKNV IMFLGDGMGV STVTAARILK GQLHHNPGEE TRLEMDKFPF VALSKTYNTN AQVPDSAGTA TAYLCGVKAN EGTVGVSAAT ERSRCNTTQG NEVTSILRWA KDAGKSVGIV TTTRVNHATP SAAYAHSADR DWYSDNEMPP EALSQGCKDI AYQLMHNIRD IDVIMGGGRK YMYPKNKTDV EYESDEKARG TRLDGLDLVD TWKSFKPRYK HSHFIWNRTE LLTLDPHNVD YLLGLFEPGD MQYELNRNNV TDPSLSEMVV VAIQILRKNP KGFFLLVEGG RIDHGHHEGK AKQALHEAVE MDRAIGQAGS LTSSEDTLTV VTADHSHVFT FGGYTPRGNS IFGLAPMLSD TDKKPFTAIL YGNGPGYKVV GGERENVSMV DYAHNNYQAQ SAVPLRHETH GGEDVAVFSK GPMAHLLHGV HEQNYVPHVM AYAACIGANL GHCAPAS |
Expression System |
HEK293 |
Predicted nterm |
Asp |
Manufacturers Product Type |
Recombinant Protein |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.