Comparison

DENV2 (strain Thailand/16681/1984) Envelope Protein, His Tag (MALS & SPR verified) European Partner

Item no. ENN-D5243-50ug
Manufacturer ACROBiosystems
Amount 50 ug
Category
Type Proteins Recombinant
Format Powder
Specific against other
Host HEK293
Conjugate/Tag Unconjugated - N-10×His
Purity 90%
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Available
Manufacturers Type
Protein
Manufacturers Category
Viral Proteins
Description
DENV2 (strain Thailand/16681/1984) Envelope Protein, His Tag (ENN-D5243) is expressed from human 293 cells (HEK293). It contains AA Phe 115 - Thr 280 & Met 281 - Ser 677 (Accession # P29990).
Background
Dengue is a mosquito-borne viral disease widely spread all over the world transmitted by 4 serotypes of dengue virus (DENV). The symptoms range from mild dengue fever to dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) with high mortality. Currently, most of the studies on DENV vaccines emphasize on its envelope protein which is essential for viral attachment and fusion (i.e., Sanofi’s tetravalent dengue vaccine Dengvaxia). However, the vaccine of envelope protein still needs to be improved to maximize its efficiency on people never infected with DENV before, which requires more research to be conducted on the DENV envelope protein.
Characteristics
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 65.0 kDa. The protein migrates as 60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Molecule
Envelope protein
Species Reactivity
Dengue virus
Exp Region
Phe 115 - Thr 280 & Met 281 - Ser 677
Buffer
PBS, pH7.4
Storage
-20℃
Shipping
RT
Molecular Weight
65.0 kDa
Endotoxin
1.0 EU per μg
Stability
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 ug
Available: In stock
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