Item no. |
BOS-PB9081-10ug |
Manufacturer |
Boster
|
Amount |
10ug |
Format |
Lyophilized |
Applications |
WB, FC, IF, IHC, ICC |
Specific against |
Human |
Host |
Rabbit |
Isotype |
IgG |
Alias |
Galectin-3;Gal-3;35 kDa lectin;Carbohydrate-binding protein 35;CBP 35;Galactose-specific lectin 3;Galactoside-binding protein;GALBP;IgE-binding protein;L-31;Laminin-binding protein;Lectin L-29;Mac-2 antigen;LGALS3;MAC2; |
Available |
|
Manufacturers Product Category |
Primary Antibodies, Rabbit Polyclonal Antibodies |
Manufacturers Research Category |
Cancer, Cell Cycle, Cell Division, Oncoproteins, Tumor Biomarkers |
Short Description |
Boster Bio Anti-Galectin 3/LGALS3 Antibody Picoband™ catalog # PB9081. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. |
Description |
Boster Bio Anti-Galectin 3/LGALS3 Antibody Picoband™ catalog # PB9081. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. |
Background |
Galectin-3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, it located on chromosome 14, locus q21– q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes. |
Gene Name |
LGALS3 |
Application Details |
Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Application Notes |
WB: The detection limit for Galectin 3 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Clonality |
Polyclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen |
E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequences identity with mouse and rat Galectin 3, respectively. |
Purification |
Immunogen affinity purified. |
Cross-reactivity |
No cross-reactivity with other proteins |
Storage |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene Full Name |
Galectin-3 |
Molecular Weight |
26152 MW |
Protein Function |
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.. |
Subcellular Localization |
Cytoplasm. Nucleus. Secreted. Secreted by a non-classical secretory pathway and associates with the cell surface. |
Tissue Specificity |
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.. |
Protein Name |
Galectin-3 |
Sequence Similarities |
Contains 1 galectin domain. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.