Item no. |
BOS-M00004-2-10ug |
Manufacturer |
Boster
|
Amount |
10ug |
Format |
Lyophilized |
Applications |
WB, FC, IF, IHC, ICC |
Clone |
1F6 |
Specific against |
Human, Mouse, Rat |
Host |
Mouse |
Isotype |
IgG1 |
Alias |
Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; OK/SW-cl.35, PRO2286 |
Available |
|
Manufacturers Product Category |
Primary Antibodies, Mouse Monoclonal Antibodies |
Manufacturers Research Category |
Actin, etc., Atherosclerosis, Cadherins, Cancer, Cardiovascular, Cell Adhesion, Cytoplasmic, Cytoskeleton, Cytoskeleton/ECM, Invasion/Microenvironment, Microfilaments, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction, Signaling Pathways, Stem Cells, Vascular Inflammation |
Short Description |
Boster Bio Anti-beta Catenin CTNNB1 Antibody Picoband™ (monoclonal, 1F6) catalog # M00004-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-beta Catenin CTNNB1 Antibody Picoband™ (monoclonal, 1F6) catalog # M00004-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Background |
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer. |
Gene Name |
CTNNB1 |
Application Details |
Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells |
Clonality |
Monoclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen |
E. coli-derived human beta Catenin recombinant protein (Position: A2-K233). |
Cross-reactivity |
No cross-reactivity with other proteins. |
Storage |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene Full Name |
catenin beta 1 |
Molecular Weight |
95 kDa |
Protein Function |
Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). |
Subcellular Localization |
Cytoplasm. Nucleus |
Tissue Specificity |
Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). |
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