Anti-POLR3F Antibody

Primary Antibodies

Boster Bio Anti-POLR3F Antibody (Catalog # A12720). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

The human POLR3F is a component of RNA III polymerase. RNA polymerase III transcribes many essential, small, noncoding RNAs, including the 5S rRNAs and tRNAs. While most pol III-transcribed genes are found scattered throughout the linear chromosome maps or in multiple linear clusters, there is increasing evidence that many of these genes prefer to be spatially clustered, often at or near the nucleolus. This association could create an environment that fosters the coregulation of transcription by pol III with transcription of the large ribosomal RNA repeats by RNA polymerase I (pol I) within the nucleolus. Given the high number of pol III-transcribed genes in all eukaryotic genomes, the spatial organization of these genes is likely to affect a large portion of the other genes in a genome. POLR3F has also been recently identified as an HIV dependency factor (HDF), suggesting that POLR3F may be an important drug target in HIV treatment. At least two isoforms of POLR3F are known to exist.

Polyclonal

POLR3F Antibody is supplied in PBS containing 0.02% sodium azide.

POLR3F antibody can be used for detection of POLR3F by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

POLR3F Antibody is affinity chromatography purified via peptide column.

Polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa

DNA-dependent RNA polymerase catalyzes the transcription
of DNA into RNA using the four ribonucleoside triphosphates as
substrates. Specific peripheric component of RNA polymerase III
which synthesizes small RNAs, such as 5S rRNA and tRNAs. May
direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key
role in sensing and limiting infection by intracellular bacteria
and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved
in innate immune response. Can sense non-self dsDNA that serves as
template for transcription into dsRNA. The non-self RNA polymerase
III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs)
induce type I interferon and NF- Kappa-B through the RIG-I
pathway. Preferentially binds double-stranded DNA (dsDNA)
(PubMed:21358628).

Ubiquitous. Up-regulated in oral cancers.