Item no. |
BOS-A12720 |
Manufacturer |
Boster
|
Amount |
0.1mg |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
Alias |
RPC6, RPC39, DNA-directed RNA polymerase III subunit RPC6, DNA-directed RNA polymerase III subunit F, RNA polymerase III subunit C6 |
Available |
|
Manufacturers Product Category |
Primary Antibodies |
Manufacturers Research Category |
Infectious Diseases |
Short Description |
Boster Bio Anti-POLR3F Antibody (Catalog # A12720). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-POLR3F Antibody (Catalog # A12720). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat. |
Background |
The human POLR3F is a component of RNA III polymerase. RNA polymerase III transcribes many essential, small, noncoding RNAs, including the 5S rRNAs and tRNAs. While most pol III-transcribed genes are found scattered throughout the linear chromosome maps or in multiple linear clusters, there is increasing evidence that many of these genes prefer to be spatially clustered, often at or near the nucleolus. This association could create an environment that fosters the coregulation of transcription by pol III with transcription of the large ribosomal RNA repeats by RNA polymerase I (pol I) within the nucleolus. Given the high number of pol III-transcribed genes in all eukaryotic genomes, the spatial organization of these genes is likely to affect a large portion of the other genes in a genome. POLR3F has also been recently identified as an HIV dependency factor (HDF), suggesting that POLR3F may be an important drug target in HIV treatment. At least two isoforms of POLR3F are known to exist. |
Gene Name |
POLR3F |
Clonality |
Polyclonal |
Concentration |
1 mg/mL |
Contents |
POLR3F Antibody is supplied in PBS containing 0.02% sodium azide. |
Predicted Reactivity |
Bovine |
Application Details |
POLR3F antibody can be used for detection of POLR3F by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher. |
Immunogen |
POLR3F antibody was raised against a 21 amino acid synthetic peptide from near the amino terminus of human POLR3F. The immunogen is located within the first 50 amino acids of POLR3F. |
Purification |
POLR3F Antibody is affinity chromatography purified via peptide column. |
Storage |
POLR3F antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Gene Full Name |
Polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa |
Molecular Weight |
35684 MW |
Protein Function |
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Preferentially binds double-stranded DNA (dsDNA) (PubMed:21358628). |
Subcellular Localization |
Nucleus. |
Tissue Specificity |
Ubiquitous. Up-regulated in oral cancers. |
Protein Name |
Polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.