Item no. |
BOS-A11286 |
Manufacturer |
Boster
|
Amount |
0.1mg |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
Alias |
AIFL, AIFL, Apoptosis-inducing factor 3, Apoptosis-inducing factor-like protein |
Available |
|
Manufacturers Product Category |
Primary Antibodies |
Manufacturers Research Category |
Apoptosis |
Short Description |
Boster Bio Anti-Apoptosis-inducing factor 3 AIFM3 Antibody (Catalog # A11286). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-Apoptosis-inducing factor 3 AIFM3 Antibody (Catalog # A11286). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat. |
Background |
Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. Recently a protein similar to the apoptosis-inducing factor (AIF) was cloned and designated AIFL (also known as AIFM3). AIFM3 is expressed ubiquitously and is predominantly localized to the inner membranes of mitochondria. Unlike AIF, AIFM3 does not translocate to the nucleus upon induction of apoptosis. However, overexpression of AIFM3, like AIF, induced cytochrome c release from the mitochondria, cleavage of caspase 3, and ultimately apoptosis, indicating AIFM3 induces apoptosis through caspase activation. Multiple isoforms of AIFM3 are known to exist. |
Gene Name |
AIFM3 |
Clonality |
Polyclonal |
Concentration |
1 mg/mL |
Contents |
AIFM3 Antibody is supplied in PBS containing 0.02% sodium azide. |
Application Details |
AIFM3 antibody can be used for the detection of AIFM3 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher. |
Immunogen |
AIFM3 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human AIFM3. The immunogen is located within the first 50 amino acids of AIFM3. |
Purification |
AIFM3 Antibody is affinity chromatography purified via peptide column. |
Storage |
AIFM3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Gene Full Name |
Apoptosis-inducing factor, mitochondrion-associated, 3 |
Molecular Weight |
66791 MW |
Protein Function |
Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential. |
Subcellular Localization |
Mitochondrion |
Tissue Specificity |
Ubiquitous. Expressed in bone marrow, cerebral cortex, liver, ovary, thymus, thyroid gland and tongue (at protein level). |
Protein Name |
Apoptosis-inducing factor, mitochondrion-associated, 3 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.