Item no. |
BOS-A10097 |
Manufacturer |
Boster
|
Amount |
0.1mg |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
Alias |
DPR2, DAPPER2, C6orf116, bA503C24.7, PP13671, Dapper homolog 2, Dapper antagonist of catenin 2 |
Available |
|
Manufacturers Product Category |
Primary Antibodies |
Manufacturers Research Category |
Signal Transduction |
Short Description |
Boster Bio Anti-Dapper homolog 2 Dact2 Antibody (Catalog # A10097). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-Dapper homolog 2 Dact2 Antibody (Catalog # A10097). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat. |
Background |
The Wnt signaling cascade is a conserved process in multicellular animals that plays important roles during development and can contribute to cancer and other diseases. Many members of this pathway are also expressed in the postnatal tissues such as brain. One such protein is Dact2, a member of the Dact protein family that was initially identified through binding to Disheveled (Dvl), a cytoplasmic protein essential to Wnt signaling. Dact2 is most prominent during the development of the thymus kidneys, and salivary gland. Dact2 is thought to play a role distinct from that of Dact1 with Dact2 having a greater impact on a beta-catenin-independent process termed planar cell polarity/convergent-extension signaling. Furthermore, Dact2 but not Dact1 can inhibit Nodal signaling by promoting the endocytic degradation of TGF-beta receptors. At least two isoforms of Dact2 are known to exist. |
Gene Name |
DACT2 |
Clonality |
Polyclonal |
Concentration |
1 mg/mL |
Contents |
Dact2 Antibody is supplied in PBS containing 0.02% sodium azide. |
Application Details |
Dact2 antibody can be used for detection of Dact2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher. |
Immunogen |
Dact2 antibody was raised against a 12 amino acid synthetic peptide from near the amino terminus of human DACT2. The immunogen is located within amino acids 150 - 200 of Dact2. |
Purification |
Dact2 Antibody is affinity chromatography purified via peptide column. |
Storage |
Dact2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Gene Full Name |
Dapper, antagonist of beta-catenin, homolog 2 (Xenopus laevis) |
Molecular Weight |
82700 MW |
Protein Function |
Involved in regulation of intracellular signaling pathways during development. Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors, such as TGFBR1. May be involved in control of the morphogenetic behavior of kidney ureteric bud cells by keeping cells epithelial and restraining their mesenchymal character. May play an inhibitory role in the re-epithelialization of skin wounds by attenuating TGF-beta signaling (By similarity).. |
Subcellular Localization |
Cytoplasm |
Tissue Specificity |
Highest levels found in fetal brain and testis. Also expressed in thymus, salivary gland and uterus. Highly expressed in cells of the innate immune system, in particular activated NK cells. Week expression in the intestine. |
Protein Name |
Dapper, antagonist of beta-catenin, homolog 2 (Xenopus laevis) |
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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.