Item no. |
BOS-A09742 |
Manufacturer |
Boster
|
Amount |
0.1mg |
Format |
Liquid |
Applications |
WB, IF, ICC, ELISA |
Specific against |
Human |
Host |
Rabbit |
Isotype |
IgG |
Alias |
HIG1, RCF1a, HIG1, HSPC010, HIG1 domain family member 1A, mitochondrial, Hypoxia-inducible gene 1 protein |
Available |
|
Manufacturers Product Category |
Primary Antibodies |
Manufacturers Research Category |
Cancer, Cell Cycle |
Short Description |
Boster Bio Anti-HIG1 HIGD1A Antibody (Catalog # A09742). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human. |
Description |
Boster Bio Anti-HIG1 HIGD1A Antibody (Catalog # A09742). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human. |
Background |
HIG1 and HIG2 (Hypoxia-inducible gene 1 and 2, respectively) are known to be induced by hypoxic conditions. HIG1 is induced by hypoxia and by glucose deprivation in cultured cells. In addition, tumor xenografts derived from human cervical cancer cells display increased expression of HIG1 and HIG2 when they are deprived of oxygen. Unlike HIG2, which is ubiquitously expressed and might be an activator and target of the canonical Wnt pathway, the function and the mechanisms underlying its regulation of HIG1 still remained unknown. The putative link between hypoxia and an oncogenic signaling pathway might play an important role in tumorigenesis. |
Gene Name |
HIGD1A |
Clonality |
Polyclonal |
Concentration |
1 mg/mL |
Contents |
HIG1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Predicted Reactivity |
Mouse, Rat |
Application Details |
HIG1 antibody can be used for detection of HIG1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher. |
Immunogen |
HIG1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human HIG1. The immunogen is located within the first 50 amino acids of HIG1. |
Cross-reactivity |
HIG1 antibody is predicted to not cross-react with HIG2 |
Purification |
HIG1 Antibody is affinity chromatography purified via peptide column. |
Storage |
HIG1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Gene Full Name |
HIG1 hypoxia inducible domain family, member 1A |
Molecular Weight |
10143 MW |
Protein Function |
Proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes. |
Subcellular Localization |
Mitochondrion membrane |
Protein Name |
HIG1 hypoxia inducible domain family, member 1A |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.