Item no. |
BOS-A05449 |
Manufacturer |
Boster
|
Amount |
0.1mg |
Format |
Liquid |
Applications |
WB, IF, ICC, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
Alias |
ULF, TRIP-12, KIAA0045, ULF, E3 ubiquitin-protein ligase TRIP12, E3 ubiquitin-protein ligase for Arf |
Available |
|
Manufacturers Product Category |
Primary Antibodies |
Manufacturers Research Category |
Homeostasis, Stem Cells |
Short Description |
Boster Bio Anti-TRIP12 Antibody (Catalog # A05449). Tested in ELISA, WB, IHC-P, IF, ICC applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-TRIP12 Antibody (Catalog # A05449). Tested in ELISA, WB, IHC-P, IF, ICC applications. This antibody reacts with Human, Mouse, Rat. |
Background |
Thyroid hormone receptors (TRs) are transcription factors that regulate the expression of specific genes in a hormone-dependent manner (1). TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent E3 ubiquitin ligase involved in the human ubiquitin fusion degradation (UFD) pathway and also modulates the NEDD8 pathway (2, 3). TRIP12 contains one WWE domain and a single HECT (E6AP-type E3 ubiquitin-protein ligase) domain suggested to contain a noncovalent ubiquitin-binding site (4). TRIP12 acts as a key regulator of DNA damage response and the ubiquitin ligase activity of TRIP12 is essential for mouse development (5). |
Gene Name |
TRIP12 |
Clonality |
Polyclonal |
Concentration |
1 mg/mL |
Contents |
TRIP12 antibody is supplied in PBS containing 0.02% sodium azide. |
Predicted Reactivity |
Bovine |
Application Details |
TRIP12 antibody can be used for detection of TRIP12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunocytochemistry start at 2 μg/mL.For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples; Immunocytochemistry in human samples; and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher. |
Immunogen |
TRIP12 antibody was raised against a 18 amino acid peptide near the carboxy terminus of human TRIP12. The immunogen is located within amino acids 1930 - 1980 of TRIP12. |
Cross-reactivity |
TRIP12 antibody is human, mouse and rat reactive. At least four isoforms are known to exist. |
Purification |
TRIP12 antibody is affinity chromatography purified via peptide column. |
Storage |
TRIP12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. |
Gene Full Name |
Thyroid hormone receptor interactor 12 |
Molecular Weight |
220434 MW |
Protein Function |
E3 ubiquitin-protein ligase involved in ubiquitin fusion degradation (UFD) pathway and regulation of DNA repair. Part of the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination of protein at their N-terminus, regardless of the presence of lysine residues in target proteins. In normal cells, mediates ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A, a lysine-less tumor suppressor required for p53/TP53 activation under oncogenic stress. In cancer cells, however, isoform p19ARF/ARF and TRIP12 are located in different cell compartments, preventing isoform p19ARF/ARF ubiquitination and degradation. Does not mediate ubiquitination of isoform p16- INK4a of CDKN2A. Also catalyzes ubiquitination of NAE1 and SMARCE1, leading to their degradation. Ubiquitination and degradation of target proteins is regulated by interaction with proteins such as MYC, TRADD or SMARCC1, which disrupt the interaction between TRIP12 and target proteins. Acts as a key regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes. |
Subcellular Localization |
Nucleus, nucleoplasm |
Protein Name |
Thyroid hormone receptor interactor 12 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.