Item no. |
BOS-A04776-2-10ug |
Manufacturer |
Boster
|
Amount |
10ug |
Format |
Lyophilized |
Applications |
WB, FC, IF, IHC, ICC, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Available |
|
Manufacturers Product Category |
Primary Antibodies, Rabbit Polyclonal Antibodies |
Short Description |
Boster Bio Anti-HADHB Antibody Picoband™ catalog # A04776-2. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry (Intracellular) applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-HADHB Antibody Picoband™ catalog # A04776-2. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry (Intracellular) applications. This antibody reacts with Human, Mouse, Rat. |
Background |
Trifunctional enzyme subunit beta, mitochondrial (TP-beta) also known as 3-ketoacyl-CoA thiolase, acetyl-CoA acyltransferase, or beta-ketothiolase is an enzyme that in humans is encoded by the HADHB gene. This gene encodes the beta subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the beta subunit catalyzing the 3-ketoacyl-CoA thiolase activity. The encoded protein can also bind RNA and decreases the stability of some mRNAs. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. Mutations in this gene result in trifunctional protein deficiency. Alternatively spliced transcript variants encoding different isoforms have been described. |
Gene Name |
HADHB |
Application Details |
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat Immunohistochemistry, 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Intracellular), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, Human |
Application Notes |
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Clonality |
Polyclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. |
Immunogen |
E.coli-derived human HADHB recombinant protein (Position: A18-H379). Human HADHB shares 91.2% and 85.9% amino acid (aa) sequence identity with mouse and rat HADHB, respectively. |
Purification |
Immunogen affinity purified. |
Storage |
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Reconstitution |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Molecular Weight |
45 kDa |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.