Item no. |
BOS-A04641-10ug |
Manufacturer |
Boster
|
Amount |
10ug |
Format |
Lyophilized |
Applications |
WB, ELISA |
Specific against |
Human |
Host |
Rabbit |
Available |
|
Manufacturers Product Category |
Primary Antibodies, Rabbit Polyclonal Antibodies |
Short Description |
Boster Bio Anti-SMPD3 Antibody Picoband™ catalog # A10547-2. Tested in WB, ELISA applications. This antibody reacts with Human. |
Description |
Boster Bio Anti-SMPD3 Antibody Picoband™ catalog # A10547-2. Tested in WB, ELISA applications. This antibody reacts with Human. |
Background |
Sphingomyelin phosphodiesterase 3 is an enzyme that in humans is encoded by the SMPD3 gene. SMPD3 (Sphingomyelin phosphodiesterase 3; also Neutral sphingomyelinase 2/nSMase2) is a 69-74 kDa member of the neutral sphingomyelinase family of enzymes. It is a monomeric Golgi/plasma membrane enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide and phosphorylcholine. This generates second messenger components that participate in signal transduction. Human SMPD3 is a two transmembrane, 655 amino acids molecule. It contains an N-terminal luminal segment (aa 1-10), a cytoplasmic region (aa 32-64), and one catalytic domain (aa 340-646). Phosphorylation increases its MW to 78 kDa in SDS-PAGE. There is one potential isoform that possesses an Asn substitution for aa 569-587. Over aa 2-655, human SMPD3 shares 91% aa sequence identity with mouse SMPD3. |
Gene Name |
SMPD3 |
Application Details |
Western blot, 0.25-0.5 μg/ml, Human ELISA, 0.1-0.5 μg/ml, Human |
Application Notes |
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Clonality |
Polyclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. |
Immunogen |
E.coli-derived human SMPD3 recombinant protein (Position: F184-D553). Human SMPD3 shares 88.9% amino acid (aa) sequence identity with both mouse and rat SMPD3. |
Purification |
Immunogen affinity purified. |
Storage |
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Reconstitution |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Molecular Weight |
75 kDa |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.