Item no. |
BOS-A03757 |
Manufacturer |
Boster
|
Amount |
0.1mg |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
Alias |
mATG9, APG9L1, MGD3208, Autophagy-related protein 9A, APG9-like 1 |
Available |
|
Manufacturers Product Category |
Primary Antibodies |
Manufacturers Research Category |
Autophagy |
Short Description |
Boster Bio Anti-Autophagy-related protein 9A ATG9A Antibody (Catalog # A03757). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-Autophagy-related protein 9A ATG9A Antibody (Catalog # A03757). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat. |
Background |
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9A, a multi-spanning membrane protein localizing to the Golgi apparatus and late endosomes, has been proposed to mediate membrane transport to generate autophagosomes. ATG9A has also been implicated as a regulator of STING (stimulator of interferon genes)-mediated innate immune response. |
Gene Name |
ATG9A |
Clonality |
Polyclonal |
Concentration |
1 mg/mL |
Contents |
ATG9A Antibody is supplied in PBS containing 0.02% sodium azide. |
Predicted Reactivity |
Bovine |
Application Details |
ATG9A antibody can be used for detection of ATG9A by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher. |
Immunogen |
ATG9A antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ATG9A. The immunogen is located within amino acids 720 - 770 of ATG9A. |
Purification |
ATG9A Antibody is affinity chromatography purified via peptide column. |
Storage |
ATG9A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Gene Full Name |
ATG9 autophagy related 9 homolog A (S. cerevisiae) |
Molecular Weight |
94447 MW |
Protein Function |
Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H. |
Subcellular Localization |
Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation- induced redistribution depends on ULK1, ATG13, as well as SH3GLB1. |
Protein Name |
ATG9 autophagy related 9 homolog A (S. cerevisiae) |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.