Anti-JMJD6 Antibody

Primary Antibodies

Boster Bio Anti-JMJD6 Antibody (Catalog # A03690-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. JMJD6 was initially identified as a membrane protein that participates in phagocytosis but recent studies have shown that it has other functions when it is expressed in the cytosol and nucleus. JMJD6 is thought to play important roles in regulation of development and differentiation as knockdown experiments in mice resulted in neonatal lethality with severe defects in the morphology of numerous organs. JMJD6 also can catalyze the lysyl-hydroxylation of U2AF65, a protein involved with RNA splicing, suggesting that some of the functions attributed to JMJD6 may be due to its regulatory activity of RNA splicing.

Polyclonal

JMJD6 Antibody is supplied in PBS containing 0.02% sodium azide.

JMJD6 antibody can be used for detection of JMJD6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

JMJD6 Antibody is affinity chromatography purified via peptide column.

Jumonji domain containing 6

Dioxygenase that can both act as a histone arginine
demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase
that catalyzes 5-hydroxylation on specific lysine residues of
target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a
regulator of RNA splicing by mediating 5-hydroxylation of
U2AF2/U2AF65, affecting the pre-mRNA splicing activity of
U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase
activity, may act as an RNA hydroxylase, as suggested by its
ability to bind single strand RNA. Also acts as an arginine
demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and
histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone
code. However, histone arginine demethylation may not constitute
the primary activity in vivo. Has no histone lysine demethylase
activity. Required for differentiation of multiple organs during
embryogenesis. Acts as a key regulator of hematopoietic
differentiation: required for angiogenic sprouting by regulating
the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be
necessary for the regulation of macrophage cytokine responses.

Highly expressed in the heart, skeletal muscle
and kidney. Expressed at moderate or low level in brain, placenta,
lung, liver, pancreas, spleen, thymus, prostate, testis and ovary.
Up-regulated in many patients with chronic pancreatitis. Expressed
in nursing thymic epithelial cells.