Item no. |
BOS-A03614 |
Manufacturer |
Boster
|
Amount |
0.1mg |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
Alias |
IFI1, IRGM1, LRG47, LRG-47, IFI1, Immunity-related GTPase family M protein, Immunity-related GTPase family M protein 1 |
Available |
|
Manufacturers Product Category |
Primary Antibodies |
Manufacturers Research Category |
Autophagy |
Short Description |
Boster Bio Anti-IRGM Antibody (Catalog # A03614). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-IRGM Antibody (Catalog # A03614). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat. |
Background |
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Two of the strongest hits implicate genes IRGM and ATG16L1, which encode proteins thought to be critical to the autophagy pathway and being significantly associated with Crohn's disease. IRGM induces autophagy and generates large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. In mouse, IRGM belongs to a family of gamma-interferon-induced GTP-binding proteins of approximately 48 kDa that also includes IRGM2 and IRGM3; this antibody may also recognize these proteins. |
Gene Name |
IRGM |
Clonality |
Polyclonal |
Concentration |
1 mg/mL |
Contents |
IRGM Antibody is supplied in PBS containing 0.02% sodium azide. |
Application Details |
IRGM antibody can be used for detection of IRGM by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher. |
Immunogen |
IRGM antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human IRGM. The immunogen is located within amino acids 30 - 80 of IRGM. |
Purification |
IRGM Antibody is affinity chromatography purified via peptide column. |
Storage |
IRGM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Gene Full Name |
Immunity-related GTPase family, M |
Molecular Weight |
20142 MW |
Protein Function |
Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility (By similarity). |
Subcellular Localization |
Golgi apparatus membrane. Cell membrane. Cytoplasmic vesicle, phagosome membrane . Cytoplasmic vesicle, autophagosome membrane . Cell projection, phagocytic cup. Behaves like an integral membrane protein. Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes. Preferentially associated with cis- and medial-Golgi.. |
Tissue Specificity |
Widely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes. |
Protein Name |
Immunity-related GTPase family, M |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.