Item no. |
BOS-A01844-2-10ug |
Manufacturer |
Boster
|
Amount |
10ug |
Format |
Lyophilized |
Applications |
WB, FC, IF, IHC, ICC |
Specific against |
Human |
Host |
Rabbit |
Isotype |
IgG |
Available |
|
Manufacturers Product Category |
Primary Antibodies, Rabbit Polyclonal Antibodies |
Short Description |
Boster Bio Anti-Nogo A/RTN4 Antibody Picoband™ catalog # A01844-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. |
Description |
Boster Bio Anti-Nogo A/RTN4 Antibody Picoband™ catalog # A01844-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. |
Background |
Human neurite outgrowth inhibitor (NOGO) cDNAs encodes 3 splice variants: NOGOA, NOGOB and NOGOC. The longest cDNA, designated NOGOA, has an open reading frame of 1192 amino acids. It is a potent inhibitor of neurite growth and an IN-1 antigen produced by oligodendrocytes, and may allow the generation of new reagents to enhance CNS regeneration and plasticity. Nogo-A, a member of the Reticulon family, is expressed by oligodendrocytes and associates primarily with the endoplasmic reticulum. The acidic amino terminus of Nogo-A is detected at the cytosolic face of cellular membranes and may contribute to inhibition of axon regeneration at sites of oligodendrocyte injury. A multivalent form of the N terminus of Nogo-A affects the morphology of both neurons and other cell types. |
Gene Name |
RTN4 |
Application Details |
Western blot, 0.1-0.25μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, human Immunocytochemistry/Immunofluorescence, 4μg/ml, Human Immunofluorescence, 4μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Application Notes |
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Clonality |
Polyclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3. |
Immunogen |
A synthetic peptide corresponding to a sequence at the N-terminus of human Nogo A/RTN4. |
Purification |
Immunogen affinity purified. |
Storage |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Molecular Weight |
180 kDa |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.