Item no. |
BOS-A01463-1 |
Manufacturer |
Boster
|
Amount |
0.1mg |
Format |
Liquid |
Applications |
WB, IF, ICC, ELISA |
Specific against |
Human, Mouse |
Host |
Rabbit |
Isotype |
IgG |
Alias |
Rap, Raptor, mKIAA1303, 4932417H02Rik, Regulatory-associated protein of mTOR |
Available |
|
Manufacturers Product Category |
Primary Antibodies |
Manufacturers Research Category |
Signal Transduction, Neuroscience |
Short Description |
Boster Bio Anti-Raptor Rptor Antibody (Catalog # A01463-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse. |
Description |
Boster Bio Anti-Raptor Rptor Antibody (Catalog # A01463-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse. |
Background |
The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation. These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels. Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif; mutation of this motif prevents the TOR-dependent phosphorylation of these proteins. |
Gene Name |
RPTOR |
Clonality |
Polyclonal |
Concentration |
1 mg/mL |
Contents |
Raptor Antibody is supplied in PBS containing 0.02% sodium azide. |
Application Details |
Raptor antibody can be used for the detection of Raptor by Western blot at 2 and 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL. Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher. |
Immunogen |
Raptor polyclonal antibody was raised against a 13 amino acid synthetic peptide from near the amino-terminus of human Raptor. The immunogen is located within amino acids 90 - 140 of Raptor. |
Cross-reactivity |
Raptor has multiple isoforms that may also be recognized by antibody. |
Purification |
Raptor Antibody is affinity chromatography purified via peptide column. |
Storage |
Raptor antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Gene Full Name |
Regulatory associated protein of MTOR, complex 1 |
Molecular Weight |
149471 MW |
Protein Function |
Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis. |
Subcellular Localization |
Cytoplasm. Lysosome . Cytoplasmic granule |
Tissue Specificity |
Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. |
Protein Name |
Regulatory associated protein of MTOR, complex 1 |
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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.