Anti-Raptor Rptor Antibody

Primary Antibodies

Boster Bio Anti-Raptor Rptor Antibody (Catalog # A01463-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation. These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels. Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif; mutation of this motif prevents the TOR-dependent phosphorylation of these proteins.

Polyclonal

Raptor Antibody is supplied in PBS containing 0.02% sodium azide.

Raptor polyclonal antibody was raised against a 13 amino acid synthetic peptide from near the amino-terminus of human Raptor. The immunogen is located within amino acids 90 - 140 of Raptor.

Raptor Antibody is affinity chromatography purified via peptide column.

Regulatory associated protein of MTOR, complex 1

Involved in the control of the mammalian target of
rapamycin complex 1 (mTORC1) activity which regulates cell growth
and survival, and autophagy in response to nutrient and hormonal
signals; functions as a scaffold for recruiting mTORC1 substrates.
mTORC1 is activated in response to growth factors or amino acids.
Growth factor-stimulated mTORC1 activation involves a AKT1-
mediated phosphorylation of TSC1-TSC2, which leads to the
activation of the RHEB GTPase that potently activates the protein
kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires
its relocalization to the lysosomes mediated by the Ragulator
complex and the Rag GTPases. Activated mTORC1 up-regulates protein
synthesis by phosphorylating key regulators of mRNA translation
and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and
releases it from inhibiting the elongation initiation factor 4E
(eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389',
which then promotes protein synthesis by phosphorylating PDCD4 and
targeting it for degradation. Involved in ciliogenesis.

Isoform 1, isoform 5 and isoform 7 are
expressed in a wide variety of tissues. Highest expression in
peripheral blood leukocytes, spleen, thymus and liver. Barely
detectable in brain, testis and skeletal muscle.