Anti-Integrin alpha 4 ITGA4 Antibody

Primary Antibodies

Boster Bio Anti-Integrin alpha 4 ITGA4 Antibody (Catalog # A00468). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

Polyclonal

Integrin alpha 4 Antibody is supplied in PBS containing 0.02% sodium azide.

Integrin alpha 4 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human Integrin alpha 4. The immunogen is located within the last 50 amino acids of Integrin alpha 4.

Integrin alpha 4 Antibody is affinity chromatography purified via peptide column.

Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)

Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are
receptors for fibronectin. They recognize one or more domains
within the alternatively spliced CS-1 and CS-5 regions of
fibronectin. They are also receptors for VCAM1. Integrin alpha-
4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-
4/beta-7 is also a receptor for MADCAM1. It recognizes the
sequence L-D-T in MADCAM1. On activated endothelial cells integrin
VLA-4 triggers homotypic aggregation for most VLA-4-positive
leukocyte cell lines. It may also participate in cytolytic T-cell
interactions with target cells. ITGA4:ITGB1 binds to fractalkine
(CX3CL1) and may act as its coreceptor in CX3CR1-dependent
fractalkine signaling (PubMed:23125415). ITGA4:ITGB1 binds to
PLA2G2A via a site (site 2) which is distinct from the classical
ligand-binding site (site 1) and this induces integrin
conformational changes and enhanced ligand binding to site 1
(PubMed:18635536, PubMed:25398877).

Megakaryocytes and platelets.