Item no. |
BOS-A00119-1-10ug |
Manufacturer |
Boster
|
Amount |
10ug |
Format |
Lyophilized |
Applications |
WB, FC, IF, ICC, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Available |
|
Manufacturers Product Category |
Primary Antibodies, Rabbit Polyclonal Antibodies |
Short Description |
Boster Bio Anti-CDC42 Antibody Picoband™ catalog # A00119-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-CDC42 Antibody Picoband™ catalog # A00119-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Background |
Cell division control protein 42 homolog also known as CDC42 is a protein involved in regulation of the cell cycle. In humans, CDC42 is encoded by the CDC42 gene.CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. |
Gene Name |
CDC42 |
Application Details |
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x1x106 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human |
Application Notes |
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Clonality |
Polyclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. |
Immunogen |
E.coli-derived human CDC42 recombinant protein (Position: N26-L191). |
Purification |
Immunogen affinity purified. |
Storage |
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Reconstitution |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Molecular Weight |
21 kDa |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.