Human iNOS ELISA Kit

Human

iNOS

Human iNOS ELISA Kit (A311049) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human iNOS in serum, plasma or other biological fluids. An antibody specific for iNOS has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the iNOS present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-iNOS Antibody, which also binds the iNOS present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-iNOS Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-iNOS Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of iNOS captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of iNOS in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Colorimetric

1h 30m