« Back
Home /
Partner: iQ Biosciences | Hölzel Diagnostika

iQ Biosciences

iQ Biosciences - Cellular Products and Bioservices

iQ Biosciences provides you primary cells of various species for numerous applications. Primary cells, such as peripheral blood mononuclear cells (PBMCs), splenocytes, and bone marrow cells, are frequently used as a valuable in vitro research tool. Our partner obtains human and non-human source tissues from well-respected institutions and isolate the relevant cell types through careful processing. One application field is represented by toxicity assessment, which is a requirement prior to proceeding to clinical trials with your therapeutic. Cryopreserved PBMCs are used for in vitro safety studies, such as the cytokine release assay, where the PBMCs are thawed and stimulated with your molecule prior to cytokine measurement. Another application is MoA characterization. When developing antibody therapeutics, it is critical to find out about its potential for inducing a cell-mediated immune defense such as ADCC or ADCP. Primary cells represent useful tools for in vitro studies to characterize mechanism of action of your therapeutic.

When researchers choose to work with iQ Biosciences, they get reliable testing, quality research samples and a trusted committed partner. From dedicated immunology based services to high quality biospecimens to strategic consultation and guidance, iQ Biosciences is your partner of choice.

Right now, thousands of researchers are developing new therapeutics to combat one of the world’s most devastating diseases. Drug development in research and preclinical stages is tricky to navigate. iQ Biosciences team of immunologists is qualified, expertised and want to help guide drug development efforts.

iQ Biosciences pays great attention to high quality products. iQ Biosciences is committed to operating under the highest ethical and legal standards in the acquisition of human and animal tissues used for the isolation of primary cells and other biological materials.

Understanding Peripheral Blood Mononuclear Cells (PBMCs) and their cryopreservation

Cryopreserved PBMCs provide an effective and efficient means of studying the immune system in health and disease. Proper isolation, cryopreservation, storage, and thawing techniques are crucial to maintain the viability and functional potential of lymphocytes. Cryopreservation allows for the standardization of experiments, which is essential when working with small or rare cell subsets. Using high-quality PBMCs is critical in research, and iQ Biosciences takes every necessary step to ensure that the cells they provide are of the highest quality.

Introduction to PBMCs

Peripheral Blood Mononuclear Cells (PBMCs) are white blood cells that play a crucial role in the immune system. PBMCs include a variety of lymphocytes, such as T cells, B cells, natural killer (NK) cells, and monocytes. They are isolated from fresh peripheral blood by performing a density centrifugation to remove red blood cells. PBMC isolation is performed under sterile conditions using ficoll, a high molecular weight sucrose solution from GE Healthcare. Depending on the species of whole blood, either 1.077 or 1.084 g/ml ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes.

Cryopreserved vs Freshly Isolated PBMCs

Cryopreservation of PBMCs provides a convenient and reliable method for storage and shipping of cells. For single experiments that require multiple donors, frozen PBMCs allow for uniform analysis of multiple samples simultaneously. Cryopreserved PBMCs also reduce the experimental time required for cell isolation. The use of cryopreserved PBMCs in immunological assays requires following proper isolation, cryopreservation, storage, and thawing techniques to maintain the viability and functional potential of lymphocytes. If these critical factors are taken care of, then there is no adverse effect on PBMC functionality in immunological assays.

Benefits of Cryopreserved PBMCs

There are multiple benefits of using cryopreserved PBMCs. Cryopreservation allows researchers to maintain a stock of cells, providing flexibility in the timing of experiments. By using cryopreserved cells, it is possible to perform longitudinal studies with the same donors, ensuring consistent data over time. Cryopreservation is also beneficial when working with samples that are not available in large quantities, such as rare cell subsets or pediatric samples. Furthermore, cryopreservation allows for the standardization of experiments by ensuring the same number of cells are used in each experiment.

Quality Control

Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications. The quality control steps involve confirming that the isolated cells are mononuclear, and that the cell count and viability meet the required specifications. It is essential to use high-quality cells in research, and iQ Biosciences ensures that the PBMCs they provide are of the highest quality.