Coronavirus Protein Array G2

Ascites
Hybridoma cell culture medium
Other fluids
Plasma
Purified antibodies
Purified protein
Serum

Semi-Quantitative

Ray
Bio® coronavirus protein array
Blocking Buffer
Wash Buffer I
Wash Buffer I
I
Biotin-conjugated secondary antibody
Cy3 equivalent dye conjugated streptavidin
Array accessories (including slide incubation chamber assembly, gasket, adhesive plastic strips, etc.)

Small sample volume required
High density
High sensitivity
Large dynamic range
Suitable for high-throughput assays

Recombinant coronavirus spike (S) proteins and nucleocapsid (N) proteins are spotted in triplicate on a standard glass slide (25 mm x 75 mm x 1 mm), along with positive and negative controls to monitor each incubation step. When liquid sample types, such as serum or purified antibodies, are incubated on the array, "primary" antibodies in the sample will bind to their specific target proteins. Specific primary antibody isotypes (IgG, IgM, or IgA) are then targeted by biotin-conjugated anti-IgG, IgM, or IgA secondary antibodies, respectively, that are specific for the host species (see the flow chart on the next page).
A fluorophore-conjugated streptavidin molecule is then added, which binds to the biotin on the secondary antibody. The fluorophore enables the detection of the immobilized primary antibody via fluorescence using a laser scanner. The fluorescence signal is proportional to the amount of immobilized antibody. Since each spot represents a unique protein that is known, the specific target(s) bound by the antibodies can be ascertained.

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