Item no. |
PT_40010_5ug |
Manufacturer |
Novateinbio
|
Amount |
5 ug |
Category |
|
Type |
Proteins Recombinant |
Specific against |
Human |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Beta Nerve Growth Factor Human Recombinant ( b NGF Human ) |
Similar products |
Beta Nerve Growth Factor Human Recombinant ( b NGF Human ) |
Available |
|
Description |
Nerve Growth Factor-beta Human Recombinant produced in E.Coli is a non-covalently disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2 identical 121 amino acids with a molecular weight of two 13.6 kDa polypeptide monomers.The NGF-b is purified by proprietary chromatographic techniques. |
Storage/Stability |
Lyophilized Beta-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NGF-Beta should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
Purity |
Greater than 95.0% as determined by SDS-PAGE. |
Form |
The beta-NGF protein was lyophilized from a 0.2um filtered solution containing 0.1% Trifluoroacetic Acid (TFA). |
Protein Background |
NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. |
Expression host |
Escherichia Coli. |
Reagent Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Amino acid sequence |
MSSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA. |
Biological Activity |
The ED50, calculated by its ability to stimulate proliferation of TF-1 cells and is typically 1, 000, 000units/mg. |
Solubility |
It is recommended to reconstitute the lyophilized NGF-b in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Manufacturers Category |
Neuroscience |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.