guanidinehydrochloride

Desription: Guanidine hydrochloride, the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant. Target: Others Guanidine hydrochloride is the most popular protein denaturant. Analysis of unfolding transitions by Guanidine hydrochloride provides several important parameters regarding the mechanism of conformational stability of proteins. Guanidine hydrochloride at low concentrations refolds acid-unfolds apomyoglobin and cytochrome c, stabilizing the molten globule state. Guanidine hydrochloride (> 1 M) causes co-operative unfolding of the molten globule state 1. Guanidine hydrochloride at millimolar concentrations, is able to causes efficient loss of the normally stable PSI+ element from yeast cells. 5 mM Guanidine hydrochloride in growth media cures PSI+ and other prions of yeast. 5 mM Guanidine hydrochloride significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. Guanidine hydrochloride also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase 2.