SN 6

415697-08-4

C20H22N2O5S

DMSO : 62.5 mg/mL (155.29 mM; Need ultrasonic); H2O : < 0.1 mg/mL (insoluble)

Membrane Transporter/Ion Channel

SN 6 is a selective Na⁺/Ca²⁺ exchanger (NCX) inhibitor, and inhibits ⁴⁵Ca²⁺ uptake by NCX1, NCX2, and NCX3, with IC₅₀s of 2.9, 16, and 8.6 uM, respectively. IC50 & Target: IC50:2.9 uM (NCX1), 16 uM (NCX2), 8.6 uM (NCX3)^[1] In Vitro: SN 6 is a selective Na⁺/Ca²⁺ exchanger inhibitor, which inhibits the initial rate of ⁴⁵Ca²⁺ uptake into NCX1, NCX2, and NCX3 transfectants with IC₅₀ values of 2.9 +/- 0.12, 16 +/- 1.1, and 8.6 +/- 0.27 uM. SN 6 (up to 30 uM) also less potently inhibits muscarinic acetylcholine receptor, with a higher IC₅₀ of 18 uM. SN 6 (0.3-30 uM) completely inhibits the initial rate of Na⁺_i-dependent ⁴⁵Ca²⁺ uptake into Na⁺-loaded sarcolemmal vesicles in a dose dependent manner (IC₅₀, 5.3 +/- 0.37 uM). SN 6 (0.3-10 uM) dose-dependently protects against the hypoxia/reoxygenation-induced LDH release in parental LLC-PK1 cells and NCX1 transfectants but not in K229Q transfectants^[1]. SN 6 (1-30 uM) suppresses the bidirectional outward and inward I_NCX in a concentration-dependent manner, with IC₅₀ values of 2.3 uM and 1.9 uM, respectively. SN 6 also inhibits bidirectional current (I_NCX) in a [Na⁺]i concentration-dependent manner, with IC₅₀ values of 3.4 uM, 2.3 uM, and 1.1 uM at 10 mM, 20 mM, and 30 mM [Na⁺]i, respectively^[2]. SN 6 inhibits hypoxia/reoxygenation-induced LDH release with an IC₅₀ value of 0.63 +/- 0.15 uM in NCX1 transfectants^[3].