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A431 Lysate

A431 Lysate

Item no.	NKM-ATGL0007-500ug
Manufacturer	NKMAX
Amount	500ug
Category	Western Blot Cell Lines & Cell Culture Cell Lysates
Type	Cell Lysate
Applications	WB
Specific against	other
ECLASS 10.1	32160890
ECLASS 11.0	32160890
UNSPSC	41105330
Available
Antigen Species	Human
Concentration	2mg/ml (determined by BCA assay)
Description	A431 cells were established from an epidermoid carcinoma in the skin/epidermis of an 85 year old female patient. A431 cells are used in studies of the cell cycle and cancer-associated cell signalling pathways since they express abnormally high levels of the EGFR. As such they are often used as a positive control for EGFR expression.
Formulation	Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl pH6.8, 0.4% SDS, 5% Glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Lysate QC	Western Blot analyzed by both GAPDH and Beta tubulin antibody
Preparation before usage	Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Storage	Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 500ug

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Delivery expected until 10/4/2024

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