SuperPrep II Cell Lysis & RT Kit for qPCR

Nucleic Acid Engineering/PCR/Taq and Kits

SuperPrep™ II Cell Lysis & RT Kit for qPCR (Code No. SCQ-401) consists of " Lysis Reagents" and " RT Reagents" for synthesis of cDNA templates for real-time PCR assays.
" Lysis Reagents" prepares cell lysates containing RNAs that can be used as templates for reverse transcription. " RT Reagents" contains reagents for reverse transcription, optimized for efficient cDNA synthesis from crude lysates. The synthesized cDNA can be applied to real-time PCR directly. This assay system is suitable for high-throughput assays. In the previous version, it was necessary to add Stop Solution after adding Lysis Solution. In SuperPrep™ II, cell lysates with lysis solution can be used as templates for cDNA synthesis directly. Moreover, high sensitivity detection is possible from a wide variety of mammalian cells than in the previous version. With this assay system, it is possible to synthesize template cDNA for real-time PCR from cultured cells conveniently and quickly.
SuperPrep™ II Cell Lysis Kit for qPCR (Code No. SCQ-501) is an option of " Lysis Reagents" . The cell lysate prepared by " Lysis Reagents" can be applied to one-step real-time PCR.
FEATURES

• RNA purification is not necessary.


• High-quality cDNA can be obtained from cell lysates.


• Reduction of dispersion on high-throughput assay.


• Various real-time PCR regents can be applied.