Item no. |
ABK-KTB1121-96T |
Manufacturer |
Abbkine Scientific
|
Amount |
96T |
Category |
|
Type |
Assay-Kit |
Specific against |
other |
ECLASS 10.1 |
32161090 |
ECLASS 11.0 |
32161090 |
UNSPSC |
41116126 |
Available |
|
Applications notes |
The kit provides a simple method for detecting Pyruvate Acid (PA) concentration in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, Pyruvic acid reacts with 2, 4-dinitrophenylhydrazine to generate pyruvate-2, 4-dinitrophenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 520 nm. The Pyruvate Acid (PA) present in the sample is proportional to the signal obtained. |
Kit components |
- Extraction Buffer - Chromogen A - Chromogen B - Standard |
Features & Benefits |
- Determination of Pyruvate Acid (PA) content in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. - Detailed sample preparation and result calculation methods are provided. - Simple operation steps. - Standard and standard curve line are provided. |
Usage notes |
- Do not mix or substitute reagents or materials from other kit lots or vendors. - The lowest detection limit is 1ug/mL or 1ug/g fresh weight or 10ng/mg prot. - Ensure all reagents and equipment are at the appropriate temperature before starting the assay. |
Storage instructions |
Storage at 4C and protected from light upon receipt. Kit has a storage time of 6 months form receipt. |
Shipping |
Gel pack with blue ice. |
Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background |
Pyruvate Acid (PA) is an important intermediate for the sugar metabolism in organisms and the mutual transformation of various substances in the body. It connects the three major metabolisms of glucose, fatty acids and amino acids through acetyl CoA and plays an important pivotal role. |
Alternative |
PA; Pyruvic acid |
Tag |
PA |
Description |
Abbkine CheKine Pyruvate Acid (PA) Colorimetric Assay Kit is designed for detecting PA in the sample. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.